F3 F3-200 Bionet Pilot to Industrial Bioreactor

Premium SIP bioreactor for developing and scaling new bioprocesses, capable of enabling customisable engineering designs while being a highly tested and well-documented product that is difficult to find in industrial-sized environments. The third generation of a model with advanced and reliable technologies

What is it used for?

The F3 bioreactor by Bionet is a high-quality stainless steel bioreactor designed to meet the diverse needs of process development, scale-up, and commercial production. Ideal for microbiology and cell culturing, the F3 excels in supporting a range of applications across the biopharma, agriculture, and food sectors. Its robust design ensures reliable performance and scalability, making it the perfect choice for advancing your biotech processes from initial development through to large-scale production.

Customisation and flexibility

The F3 bioreactor by BIONET is engineered for both pilot and industrial environments, combining standardized SIP technology with a professional design and construction. Available in 50, 100, and 200-liter working volumes, it comes in distinct models optimized for microbial or cell culture applications. Its extensive range of ports and Plug&Play add-ons offer exceptional configurability and expandability, adapting seamlessly to evolving projects and multipurpose plants. This versatility makes it an ideal choice for advancing bioprocesses into industrial-scale production across biopharma, agriculture, and food sectors.

Key benefits

Robustness

Ensure consistent performance with a robust product that has been thoroughly tested;
Rely on top-tier components and technologies, designed for precision and consistency in bioprocessing, ensuring quality, durability, and performance;
Ensure smooth fermentation operations through effective preventive maintenance with a recommended spare parts list for critical components and consumables;
Eliminate integration costs and risks with a single supplier for both upstream and downstream bioprocess equipment, ensuring a seamless process with Bionet’s integrated solutions.

Scalability

Ensure efficient and contamination-free production at larger volumes with an SIP system;
Maintain consistent mixing, oxygen transfer, and temperature control from small-scale to large-scale production by preserving geometric similarity;
Guarantee optimal process scalability with standard solutions identical across all units.

Flexibility

Adjust bioreactor to your specific need with more than 30 different add-ons for hundreds of possible combinations;
Adapt to evolving process requirements and technological advancements with a future-proof system featuring 6 free I/O signals, spare ports for new probes and actuators, and an expandable frame for seamless integration of add-ons;
Reduce upfront investment and allow easy expansion if needed with Plug&Play design philisophy.

User-friendliness

Simplify operation and data access with software Marta, designed with an intuitive user interface;
Facilitate maintenance and troubleshooting with ports, valves, and main components in an accessible 2D layout;
Optimise your lab space with a compact bioreactor designed for high performance and a small footprint.

MODEL

F3-50

F3-100

F3-200

F3-300

F3-500

Reactor and agitation
Max. Working volume	50 L	100 L	200 L	300 L	500 L
Vessel design	SIP jacketed stainless steel (A316L) vessel with Kloppler bottom
Agitator	Lower-mounted agitator with electrical motor and gear reducer
Seal	Single-mechanical seal (FDA compliant design). Double-mechanical seal and magnetic coupling available as an option.
Impellers	3 (6-blades) Rushton impellers (M) or Marine Impellers (C)
No. of Baffles	4
Materials	Vessel and all product-contact parts made of stainless steel A316L and borosilicate glass, rest of stainless steel A304. EPDM and silicone gaskets. Product-contact surfaces (Ra<0,8 μm) polished. Ra <0.5 μm and/or electropolishing available on demand.
Isolation	50 mm Rockwool (available as an option)
Microbiology
Total volume (M)	75 L	143 L	250 L	446 L	690 L
Working volume % (M)	67	70	70	67	73
Min. Working Volume (M)	17 L	25 L	50 L	65 L	95 L
Total H:D (M)	3:1	3:1	3:1	3:1	3:1
Working H:D (M)	1,8:1	2,3:1	2,3:1	1,9:1	2,0:1
Agitation speed (M)	100 – 700 rpm	100 – 800 rpm	100 – 800 rpm	150 – 500 rpm	150 – 500 rpm
Impeller to vessel inter- nal diameter ratio (M)	0.30-0.35
Motorpower (M)	2,2 kW	2,2 kW	3 kW	5,5 kW	5,5 kW
Bioreactor height (M)	962 mm	1206 mm	1601 mm	1782 mm	2216 mm
External diameter (M)	325 mm	406 mm	530 mm	610 mm	670 mm
Cellular
Total volume (C)	75 L	142 L	294 L	426 L	704 L
Working volume % (C)	67	70	68	70	71
Min. Working Volume (C)	20 L	32 L	62 L	77 L	118 L
Total H:D (C)	2:1	2:1	2:1	2:1	2:1
Working H:D (C)	1,2:1	1,5:1	1,5:1	1,3:1	1,3:1
Agitation speed (C)	25 -200 rpm	25 – 200 rpm	25 – 200 rpm	40 – 125 rpm	40 – 125 rpm
Impeller to vessel inter- nal diameter ratio (C)	0,4 – 0,5
Motorpower (C)	1,5 kW	1,5 kW	2,0 kW	2,2 kW	2,2 kW
Bioreactor height (C)	770 mm	985 mm	1278 mm	1501 mm	1745 mm
External diameter (C)	365 mm	450 mm	600 mm	660 mm	785 mm
Dimensions
Plant height and footprint (frame included) (mm)	1500(W) x 2197(H) x 810(D)	1500 (W) x 2273(H) x 830(D)	1500 (W) x 2653(H) x 950(D)	1625(W) x 3107 (H) x 1135(D)	1690(W) x 3420(H) x 1210(D)